About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.
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This is described in Chapter 2 Installation. Purge valve 5 Purfiier fluid starts to enter the syringe, continue to draw a few milliliters before closing the purge valve. At delivery, the mixer is delivered with a 0. Sample application technique Volume to inject Sample loop manual filling automated filling optional 0—21 ml 0.
ÄKTApurifier User Guide – GE Healthcare Life Sciences
This allows for binary gradients with high pressure mixing. When scouting over a broad pH range fine tuning is less beneficial. Please, refer to the Technical specifications for fuse data.
The curves are named as Result The seal should allow easy insertion and removal of the aktta. The pH electrode is delivered with a transparent cover. The method is held in its current status.
Store all the boxes and plastic bags in a convenient nearby place. Below is a description of how to perform a flow rate scouting. Connect the tubing by first inserting the tubing fully, then tightening the connector finger-tight.
These examples can be used as a template for zwitter ions: A number of pages appear as in the Run Setup in the Method Editor. Check that it is the correct system. Place the waste tubings marked W1, W2 and W3 in a waste bottle and place the bottle in a convenient place.
The mixer function is checked by placing a stirrer bar on top of the mixer housing. Fraction collector installed optional. The system simplifies the transition from laboratory to full scale production. Check for a dirty or broken pH electrode. The length of each block is marked at the bottom of the graph.
Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment. The peak is then collected in either one fraction volume or in several, using the Peak Fractionation Volume parameter.
Additional buttons are provided in the toolbar. Always place the buffer bottles lower than the mixer when changing chambers, to prevent draining 5. Panes from the menu to select which panes to display. When a new method is created and a recipe is selected, default correction factors will apply.
The Curves pane shows curves during the run. Normally, the curves are scaled with auto scaling i. If you cannot find your column in the list, you can add one. The subsequent Sections describe: They can also be used for changing buffer during the method run.
ÄKTApurifier User Guide – GE Healthcare Life Sciences |
The faults and actions are maanual as follows: For example, if a fraction collector is installed, options for setting up the fractionation will appear in the wizard. A 15 m UniNet cable is available as an accessory msnual should be used to connect the computer mankal the separation unit.
Do not block the ventilation inlet on the front panels and the outlets on the rear of the instrument! Highlight curves to view To return to the complete chromatogram, right-click and select Undo or Reset zoom.
The optical unit might have to be moved slightly to the left. Drop synchronization eliminates sample loss during tube change. New recipes can also be created, see Section 8. Some of the pages are briefly described in the steps below. After a while the Windows desktop appears. Mix, using a 25 ml pipette, 25 ml of the buffer stock solution, 25 ml of the acid and 50 ml of water.
Two techniques can be akha for filling the sample loop; partial or complete filling. Used for most columns. Some instructions, for example gradient or fraction instructions, might take time to complete.
The separation unit of the chromatography system has two main modules which are stacked on each other. The relevant system specifications are listed below. For full details of specifications, methods, maintenance etc. This is a base with only one pKa. The cell can be placed up to 1.