BD CaliBRITE beads are designed for use with FACSComp or AutoCOMP software and the FACS family of flow cytometers (FACSCalibur, FACSort, FACScan. values for BD Calibrite beads. To edit, see page A Target file is also created for. HLA-B Although used by. BD FACSComp software, the file is not editable. Product Name: BD CALIBRITE BEADS. Synonyms: BD CALIBRITE BEADS; CALIBRITE BEADS. CAS: MF: MW: 0. EINECS: Mol File: Mol File. BD CALIBRITE .
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BD Calibrite™ Beads
If this occurs, manually adjust the settings. Notice populations with a lower FSC signal than lymphocytes debris, for example can be excluded by increasing the FSC threshold level. Optimizing Scatter Figure 1 shows a lysed whole blood LWB sample from a normal donor before and after optimization. Optimize settings for your sample, as needed. This instructions for use IFU provides information for two- three- and four-color setup. See Figure 1 through Figure 5 for examples. NOTE Invert bead vials completely when clibrite a drop to the tube.
After the instrument settings have been determined, BD Calibrite beads are used to evaluate instrument sensitivity. All forms are provided in stabilized, buffered saline with 0. Instrument settings might need to be manually optimized before running cells.
Adjust fluorescence compensation using Tube B. Use the same staining method and run in parallel with the test samples. The FSC threshold is adjusted to a level that minimizes background signal if any.
If deterioration is suspected, prepare a new bead suspension and check instrument conditions. Gently mix the BD Calibrite bead vials, then add 1 drop of beads to each tube as indicated in the table below. NOTE Different immunophenotyping preparation methods might require different optimization procedures. Both scatter and fluorescent light signals are detected.
The drop should be cloudy, indicating the beads are properly mixed.
Next, the software adjusts fluorescence compensation using a mixed-bead suspension containing equal amounts of the appropriate BD Calibrite beads. The light scatter sensitivity is determined by the amount of channel separation between the mixed bead population and instrument background signal. Do not use after the expiration date shown on the label.
Beads used beyond their stability begin to show a decrease in separation between unlabeled and labeled populations, possibly resulting in Sensitivity Test failure. Weather and Climate for Educators.
BD Calibrite™ – BD Calibrite PerCP-Cy Beads – BD Biosciences
Optimization and Quality Control Because leucocytes have different optical properties than BD Calibrite beads, optimization of instrument settings with cell samples is important.
UV Bead lab with graph. Beads used beyond their stability begin to show a decrease in separation between unlabeled and labeled populations, resulting in Sensitivity Test failure. Do not dilute PerCP-Cy5.
A minimum channel separation must be met for the scatter and fluorescence parameters. Always refer to the appropriate application note or reagent IFU.
BD Calibrite PerCP-Cy5.5 Beads
FL1, FL2, and FL3 fluorescence sensitivity is determined by measuring the mean channel separation between the signal of the labeled beads and the unlabeled beads. The following list illustrates PMT light signal detection: Optimize instrument settings following two-color setup using a blood sample calibrife with any combination of monoclonal antibodies that identifies separate non-overlapping cell populations, such as FITClabeled and PE-labeled monoclonal antibodies.
For further assistance, contact your BD Biosciences service representative. Wellington, Auckland, New Zealand bdbiosciences. See examples in Optimization and Quality Control on page 4. Figure 1 through Figure 4 show examples of optimization bezds two- three- and four-color applications.
Following PMT and compensation adjustment, the software performs a Sensitivity Test using the appropriate mixed-bead suspension.